
PARTIAL PURIFICATION AND EFFECT OF TEMPERATURE AND HEAT STABILITY STUDIES ON RHODANESE FROM THE LIVER OF A LOCAL GOAT
ABSTRACT
The research is aimed at investigating the effect of temperature and heat stability of Rhodanese from the Liver of a local goat. This research shows the existence of rhodanese from a local goat liver homogenate. It was observed that the enzyme was partially purified by ammonium sulphate precipitation and dialysis. The specific activity of enzyme after partial purification using ammonium sulphate precipitation and dialysis was 4.39 U/mg. The effect of temperatures between 30 and 80?C on the rhodanese activity showed that optimum temperature for the enzyme was at 60?C. It was found that the enzyme (rhodanese) from a local goat liver was stable at 60?C, but was unstable at temperatures = 70oC.
CHAPTER ONE
1.1 Introduction
… This chapter will address the background information that motivated this study, the challenges that prompted it, its aim, and its objectives as a preface to subsequent sections of the study. Additional factors include the study's significance, scope, limitations, research questions and hypotheses, and the definition of technical terms.
CHAPTER TWO
2.0 Literature Review
2.1 Introduction
The review of relevant literature is the main topic of this chapter. A literature review covers theoretical and methodological contributions to a certain issue in addition to the state of knowledge at the time of writing. It provides information about the state of the art in relation to the subject you are writing about. It examines the body of work on the chosen subject. The literature evaluation in this study comprises the …
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TABLE OF CONTENTSPRELIMINARY PAGES
- Title page
- Approval page
- Dedication
- Acknowledgement
- Table of Contents
- Abstract
- 1.0 Introduction
- 1.1 Aim of research
- 2.0 Literature Review
- 2.1 Physiochemical features of rhodanese
- 2.1.1 Mechanism of action
- 2.1.2 Assay methods for rhodanese
- 2.1.3 Mechanism of rhodanese catalysis
- 2.1.4 Structure
- 2.1.5 Amino acid composition
- 2.1.6 Active sites
- 2.2 Goat
- 2.3 Liver
- 2.3.1 Synthesis
- 2.3.2 Liver disease
- 2.3.3 Fetal blood supply
- 3.0 Materials and Methods
- 3.1 Materials used
- 3.2 Reagents used
- 3.3 Preparation of buffer and reagents used
- 3.4 Collection of sample
- 3.5 Preparation of tissue extract
- 3.6 Enzyme assay
- 3.7 Protein determination
- 3.8 Effect of temperature on rhodanese
- 3.9 Purification of rhodanese from crude extract
- 3.10 Determination of protein concentration and enzyme activities
- 4.0 Results
- 5.0 Discussion, Conclusion and Recommendation
- 5.1 Discussion
- 5.2 Conclusion
- 5.3 Recommendations
REFERENCES
.
Disclaimer for Complete Material Utilization
The displayed research work titled "Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat" is stated as a research guideline towards accomplishing your assigned project / seminar research work. All the research materials on this website are ONLY for research purposes and should be used as a guideline in developing your research work. For no reason should you copy word for word as projectlist.com.ng will not be liable for any who copied the material. By ordering the complete research guideline, it signifies that you've accepted our terms of service.
PRELIMINARY PAGES
- Title page
- Approval page
- Dedication
- Acknowledgement
- Table of Contents
- Abstract
- 1.0 Introduction
- 1.1 Aim of research
- 2.0 Literature Review
- 2.1 Physiochemical features of rhodanese
- 2.1.1 Mechanism of action
- 2.1.2 Assay methods for rhodanese
- 2.1.3 Mechanism of rhodanese catalysis
- 2.1.4 Structure
- 2.1.5 Amino acid composition
- 2.1.6 Active sites
- 2.2 Goat
- 2.3 Liver
- 2.3.1 Synthesis
- 2.3.2 Liver disease
- 2.3.3 Fetal blood supply
- 3.0 Materials and Methods
- 3.1 Materials used
- 3.2 Reagents used
- 3.3 Preparation of buffer and reagents used
- 3.4 Collection of sample
- 3.5 Preparation of tissue extract
- 3.6 Enzyme assay
- 3.7 Protein determination
- 3.8 Effect of temperature on rhodanese
- 3.9 Purification of rhodanese from crude extract
- 3.10 Determination of protein concentration and enzyme activities
- 4.0 Results
- 5.0 Discussion, Conclusion and Recommendation
- 5.1 Discussion
- 5.2 Conclusion
- 5.3 Recommendations
REFERENCES
.Disclaimer for Complete Material Utilization
The displayed research work titled "Partial Purification and Effect of Temperature and Heat Stability Studies on Rhodanese From the Liver of a Local Goat" is stated as a research guideline towards accomplishing your assigned project / seminar research work. All the research materials on this website are ONLY for research purposes and should be used as a guideline in developing your research work. For no reason should you copy word for word as projectlist.com.ng will not be liable for any who copied the material. By ordering the complete research guideline, it signifies that you've accepted our terms of service.
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