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Microbiological Examination of Underground Water Source (Well-Water) At Campus 3

MICROBIOLOGICAL EXAMINATION OF UNDERGROUND WATER SOURCE (WELL-WATER) AT CAMPUS 3


ABSTRACT

The examination of underground source (well water) at campus 3 of IMT Enugu was carried out, by using ten samples of the water sample. For viable plate count for presence of pathogenic organisms and organisms indicating organic matter pollution, plating technique of poor plates were used. Also presence of coliform groups and escheriachria coli were examined using presumptive coliform, test confirmatory and completed test techniques.

The media used included nutrient agar, macconkey agar, and brilliant green bile agar, maccnokey broth and brilliant green bile broth. The total viable plate count gave 5 CFU per each for pathogenic organisms indicating organic matter pollution whereas the presumptive test showed the presence of coliform groups in eight samples out of ten samples and presence of escherichria coli in seven samples out of ten. The comfirmatory tests showed the presence of coliforms groups in six samples out of ten and presence of E. coli in four samples out of ten.

Whereas, the completed test gave the highest coliform count of 2 CFU in one sample and 1 CFU each in three samples, while only one sample out of ten gave 1 CFU for E. coli count. And the mean count for coliform, for the completed test was 0.6 CFU. The results indicated the presence of coliforms and E. coli, but the microbial load was minimal in all the tests. Therefore, the underground water source (well water) at campus 3 of IMT, Enugu is not fit for drinking though can be used for washing clothes and bathing.


CHAPTER ONE

1.1 Introduction

… This chapter will address the background information that motivated this study, the challenges that prompted it, its aim, and its objectives as a preface to subsequent sections of the study. Additional factors include the study's significance, scope, limitations, research questions and hypotheses, and the definition of technical terms.


CHAPTER TWO

2.0 Literature Review

2.1 Introduction

The review of relevant literature is the main topic of this chapter. A literature review covers theoretical and methodological contributions to a certain issue in addition to the state of knowledge at the time of writing. It provides information about the state of the art in relation to the subject you are writing about. It examines the body of work on the chosen subject. The literature evaluation in this study comprises the …


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