16S RNA IN METAGENOMIC ANALYSIS
ABSTRACT
Metagenomics is study of collective genomes of microorganisms. By using metagenomics, genomic analysis of microorganisms will be performed by direct extraction and cloning of DNA from an assemblage of microorganisms, collected from various types of environment such as soil, river, feces and marine. The aim of the study is to examine the 16s RNA in Metagenomic Analysis. In achieving this aim, the following specific objectives were laid out to perform genomic analysis of microorganisms in water, assess rapidly the environmental health condition and status in the future application, evaluate the sequence of information in assessing river water quality and study water quality can widen our perspective in assessing pollution level of the river. In total 24 isolates which are consisted of 18 isolates from polluted rivers and six from unpolluted one, were sequenced. Along these sequences, six of 16S rRNA sequences of colliform bacteria obtained from genebank were placed in Operational Taxonomic Units (OTUs). Phenetic analysis revealed that the river water used in this study were classified into two groups, representing the polluted and unpolluted. Since the tree can clearly distinct unpolluted river water from that of polluted one, we tried to develop a putative sequences motif for both condition. In practical purpose, the sequences motif can be used to screen river water quality in certain river water environment. This study will be of immense benefit to micro biologist, and other researchers who intend to know more on this study and can also be used by non-researchers to build more on their research work. This study contributes to knowledge and could serve as a guide for other study.
CHAPTER ONE
1.1 Introduction
Metagenomics, or also known as environmental genomics, is study of collective genomes of microorganisms. By using metagenomics, we are able to perform genomic analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of microorganisms, collected from various types of environment such as soil, river, feces and marine (Handelsman, 2004; Bhuiyan et al., 2011). The recent development of molecular biology designed to access this wealth of genetic information through environmental nucleic acid extraction has provided a means of avoiding the limitations of culture-dependent genetic exploitation (Cowan et al., 2005).
As a prelude to other parts of this study, this chapter will discuss the background upon which this study was initiated, the statement of problems that led to this study, the Aim and Objectives of the study. Others are Significance of the study, Scope of work, Research hypothesis and questions, Limitations of the Study and Definition of technical terms.
CHAPTER TWO
2.0 Literature Review
2.1 Introduction
The review of relevant literature is the main topic of this chapter. A literature review covers theoretical and methodological contributions to a certain issue in addition to the state of knowledge at the time of writing. It provides information about the state of the art in relation to the subject you are writing about. It examines the body of work on the chosen subject. The literature evaluation in this study comprises the …
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TABLE OF CONTENTSPRELIMINARY PAGES
- Title page
- Approval page
- Dedication
- Acknowledgement
- Table of Contents
- Abstract
INTRODUCTION
- 1.1 Introduction
- 1.2 Background …
- ⋮
LITERATURE REVIEW
- 2.1 Introduction
- ⋮
CHAPTER THREE
MATERIALS AND METHODS
- 3.1 Introduction
- ⋮
CHAPTER FOUR
RESULTS AND DISCUSSION
- 4.1 Introduction
- 4.2 Results
- ⋮
- 4.3 Discussion of Findings
CHAPTER FIVE
SUMMARY, CONCLUSION AND RECOMMENDATION
- 5.1 Introduction
- 5.2 Summary
- 5.3 Conclusion
- 5.4 Recommendation
REFERENCES
Disclaimer for Complete Material Utilization
The displayed research work titled "16s RNA in Metagenomic Analysis" is stated as a research guideline towards accomplishing your assigned project / seminar research work. All the research materials on this website are ONLY for research purposes and should be used as a guideline in developing your research work. For no reason should you copy word for word as projectlist.com.ng will not be liable for any who copied the material. By ordering the complete research guideline, it signifies that you've accepted our terms of service.
PRELIMINARY PAGES
- Title page
- Approval page
- Dedication
- Acknowledgement
- Table of Contents
- Abstract
INTRODUCTION
- 1.1 Introduction
- 1.2 Background …
- ⋮
LITERATURE REVIEW
- 2.1 Introduction
- ⋮
CHAPTER THREE
MATERIALS AND METHODS
- 3.1 Introduction
- ⋮
CHAPTER FOUR
RESULTS AND DISCUSSION
- 4.1 Introduction
- 4.2 Results
- ⋮
- 4.3 Discussion of Findings
CHAPTER FIVE
SUMMARY, CONCLUSION AND RECOMMENDATION
- 5.1 Introduction
- 5.2 Summary
- 5.3 Conclusion
- 5.4 Recommendation
REFERENCES
Disclaimer for Complete Material Utilization
The displayed research work titled "16s RNA in Metagenomic Analysis" is stated as a research guideline towards accomplishing your assigned project / seminar research work. All the research materials on this website are ONLY for research purposes and should be used as a guideline in developing your research work. For no reason should you copy word for word as projectlist.com.ng will not be liable for any who copied the material. By ordering the complete research guideline, it signifies that you've accepted our terms of service.
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