EFFECT OF METHANOLIC LEAF EXTRACT ON ACALYPHA WILKESIANA ON WEIGHT PARAMETERS IN PARACETAMOL INDUCED HEPATOXICITY IN MALE RATS

ABSTRACT

The potential effect of methanolic extract of the leaves of Acalypha wilkesiana on the weight of wistar rats was investigated. Calculated amount of methanolic leaf extract of Acalypha Wilkesiana were constituted in distilled water from the stock solution to give doses of 500,500 and 1000 mg/kg body weight of paracetamol, vitamin C and leaf extract respectively and administered to the various groups (A, B, C and D). Prior to the administration of methanolic leaf extract of Acalypha Wilkesiana at every interval of seven days, the body weights of the animals were recorded.

The acute administration of the aqueous leaf extract of Acalypha wilkesiana did not result in obvious signs of morphological changes or death of male rats throughout the experimental period. A significant difference (P>0.05) was obtained in the average body weight of animals administered with the extract as compared with the paracetamol treated group. The organ weight also elucidated no significant differences (P<0.05) in the kidney, brain, heart, lung and pancreas except for the liver where there was an increase in the weight of the liver treated with extract as compared to that of the rats treated with paracetamol. From the results obtained it is shown that the extract altered the weight of the liver and is therefore not hepatoprotective, this extract should undergo further investigation before oral administration is recommended.

CHAPTER ONE

Introduction And Literature Review

1.1 Medicinal Plants

The use of plants for healing purposes has always been part of human culture and it is getting increasingly popular in Nigeria. Acalypha wilkesiana is one of several medicinal plants used in Nigeria and it has various ethno botanical uses. Acalypha wilkesiana belongs to the family Euphorbiaceae. It is propagated by stem cuttings at any time of the year. Under ideal conditions, it grows as a spreading evergreen shrub with upright branches that tend to originate near the base and can get up to 3.1 m tall with a similar spread. It has leafs (12.7- 20.3 cm long) that are alternate, elliptic to oval, serrate and multi-coloredans small inconspicuous flowers (10.2-20.3 cm) that hangs in catkin-like racemes beneath the foliage

(Al-attar, 2010).

In some parts of southern Nigeria, the use of diuretics in the treatment of hypertension has been traditionally substituted for aqueous leaf extract of Acalypha wilkesiana. Acute changes in body mass over a short time period can frequently be assumed to be due to body water loss or gain; 1 ml of water has a mass of 1 g and therefore changes in body mass can be used to quantify water gain or loss. Over a short time period, no other body component will be lost at such a rate, making this assumption possible (Shirreffs, 2003), thus weight parameters were evaluated and used as makers of hydration status of the male Wistar rats.

1.1.1 Acalypha Specie

Kingdom; Plantae
Order; Malpighiales
Family; Euphorbiaceae
Subfamily; Acalyphoideae
Tribe; Acalypheae
Subtribe; Acalyphinae Genus Acalypha

Acalypha is a plant genus of the family Euphorbiaceae. It is the sole genus of the subtribe Acalyphinae. With 450 to 500 species of shrubs, trees and annuals, the genus is only behind Euphorbia, Croton and Phyllanthus in terms of Malpighiales diversity. The common name is copperleaf, three-seeded mercury or cat’s tail (Pax et al, 1924). These plants are mostly tropical or subtropical, with a few representatives in temperate zones. The Americas contain two thirds of the known species, distributed from southern United States to Uruguay and northern Argentina. Several species, such as Acalypha ecuadorica, Acalypha eggersii and Acalypha raivavensis are nearly extinct, and the St. Helena Mountain Bush or “stringwood” Acalypha rubrinervis already is hispida (chenille plant, red-hot cat’s tail), cultivated as a houseplant because of its colourful and texturally exciting flowers, has gained the Royal Horticultural Society’s Award of Garden Merit. (Grubben et al, 2004), as has Acalypha hispaniolae (Hispaniola cat’s tail). Others are grown for their foliage and a number of cultivars have been developed, such as Acalypha wilkesiana ‘Obovata Cristata’ and Acalypha wilkesiana Acalypha wilkesiana “Hoffmannii’. Acalypha bipartita is eaten as a vegetable in some parts of Africa.

1.1.2 Acalypha Wilkesiana

Kingdom; Plantae
Order; Malpighiales
Family; Euphorbiaceae
Genus; Acalypha
Species; Acalypha wilkesiana
Binomial name; Acalypha wilkesiana

Acalypha wilkesiana is an evergreen shrub. It grows 3 m high and spreads 2 m across. The stem is erect with many branches. The branches have fine hairs. It has a closely arranged crown. The leaves are coppery green with red splashes of colour. This gives them a mottled appearance. The leaves are large and broad with teeth around the edge. They can be 10–20 cm long and 15 cm wide. The leaves are finely hairy. They can be flat or crinkled. The flowers are reddish in spikes at the end of branches. They have separate male and female flowers on the same plant. The male flowers are in long spikes which hang downwards while the female flowers are in short spikes. They do not show up easily as they are often hidden among the leaves.

The flower stalks are 10–20 cm long. A tropical and subtropical plant which grows naturally in Vanuatu. It occurs in the Pacific Islands. It prefers light well drained soil. It suits a protected shady position. It is damaged by both drought and frost. It needs a minimum temperature above 10°C. It suits hardiness zones 9-12. Acalypha wilkesiana ointment is used to treat fungal skin diseases (Oyelami et al, 2003) carried out a non-comparative study to evaluate the safety and efficiency of Acalypha wilkesiana ointment using 32 Nigerians with mycological as well as clinical evidence of mycoses.

The ointment successfully controlled the mycoses in 73.3% of the affected patients. It was very effective in treating Pityriasis versicolor, Tinea pedia and Candida intetrigo, with 100% cure (Oyelami et al, 2003) concluded that Acalypha wilkesiana ointment can be used to treat superficial mycoses (Akinyemi et al, 2005) evaluated crude extracts from six important medicinal plants, namely: Phylantus discoideus, Ageratum conyzoides, Terminalia avicennioides, Bridella ferruginea, Acalypha wilkesiana and Ocimum gratissimum, to find activity against methicillin resistant Staphylococcus aureus (MRSA.

Water and ethanolic extracts of these plants were obtained locally. MRSA strains isolated from patients were used. Both ethanolic and water extracts showed effects on MRSA. Minimum bactericidal concentration (MBC) and minimum inhibition concentration (MIC) of these plants ranged from 30.4-37.0 mcg/ml and 18.2-24.0 mcg/ml respectively. A high MBS value was found in two plants and the other four contained traceable amounts of anthraquinones. This study provided scientific support for the use of Acalypha wilkesiana, T. avicennioides, O. gratissimum and P. discoidens against MRSA based diseases. A. conyzoides and B. ferruginea were unresponsive against the MRSA strains (Oyelami et al, 2003).

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CHAPTER TWO

2.0 Literature Review

2.1 Introduction

The review of relevant literature is the main topic of this chapter. A literature review covers theoretical and methodological contributions to a certain issue in addition to the state of knowledge at the time of writing. It provides information about the state of the art in relation to the subject you are writing about. It examines the body of work on the chosen subject. The literature evaluation in this study comprises the …

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TABLE OF CONTENTS
PRELIMINARY PAGES

Title page
Approval page
Dedication
Acknowledgement
Table of Contents
Abstract
CHAPTER ONE
INTRODUCTION
⋮
CHAPTER TWO
LITERATURE REVIEW
2.1 Introduction
⋮
CHAPTER THREE
MATERIALS AND METHODS
3.1 Introduction
⋮
CHAPTER FOUR
RESULTS AND DISCUSSION
4.1 Introduction
4.2 Results
⋮
4.3 Discussion of Findings
CHAPTER FIVE
SUMMARY, CONCLUSION AND RECOMMENDATION
5.1 Introduction
5.2 Summary
5.3 Conclusion
5.4 Recommendation
REFERENCES

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